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- Title
Phosducin-Like Protein 1 is Essential for G-Protein Assembly and Signaling in Retinal Rod Photoreceptors.
- Authors
Lai, Chun Wan J.; Kolesnikov, Alexander V.; Frederick, Jeanne M.; Blake, Devon R.; Li Jiang; Stewart, Jubal S.; Chen, Ching-Kang; Barrow, Jeffery R.; Baehr, Wolfgang; Kefalov, Vladimir J.; Willardson, Barry M.
- Abstract
G-protein ß subunits perform essential neuronal functions as part of G-protein ß? and Gß5-regulators of G-protein signaling (RGS) complexes. Both Gß? and Gß5-RGS are obligate dimers that are thought to require the assistance of the cytosolic chaperonin CCT and a cochaperone, phosducin-Iike protein 1 (PhLPl) for dimer formation. To test this hypothesis in vivo, we deleted the Phlpl gene in mouse (Mus musculus) retinal rod photoreceptor cells and measured the effects on G-protein biogenesis and visual signal transduction. In the PhLPl-depleted rods, Gß? dimer formation was decreased 50-fold, resulting in a > 10-fold decrease in light sensitivity. Moreover, a 20-fold reduction in Gß5 and RGS9-1 expression was also observed, causing a 15-fold delay in the shutoff of light responses. These findings conclusively demonstrate in vivo that PhLPl is required for the folding and assembly of both Gßy and Gß5-RGS9.
- Subjects
PHOSDUCIN; G proteins; CELLULAR signal transduction; PHOTORECEPTORS; BRAIN function localization; MOLECULAR chaperones; GENE expression
- Publication
Journal of Neuroscience, 2013, Vol 33, Issue 18, p7941
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCi.5001-12.2013