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- Title
The RNA-Binding Protein Human Antigen R Controls Global Changes in Gene Expression during Schwann Cell Development.
- Authors
Iruarrizaga-Lejarreta, Marta; Varela-Rey, Marta; Lozano, Juan José; Fernández-Ramos, David; Rodríguez-Ezpeleta, Naiara; Embade, Nieves; Lu, Shelly C.; van der Kraan, Peter M.; Blaney Davidson, Esmeralda N.; Gorospe, Myriam; Mirsky, Rhona; Jessen, Kristján R.; Aransay, Ana María; Mato, José M.; Martínez-Chantar, María L.; Woodhoo, Ashwin
- Abstract
An important prerequisite to myelination in peripheral nerves is the establishment of one-to-one relationships between axons and Schwann cells. This patterning event depends on immature Schwann cell proliferation, apoptosis, and morphogenesis, which are governed by coordinated changes in gene expression. Here, we found that the RNA-binding protein human antigen R (HuR) was highly expressed in immature Schwann cells, where genome-wide identification of its target mRNAs in vivo in mouse sciatic nerves using ribonomics showed an enrichment of functionally related genes regulating these processes. HuR coordinately regulated expression of several genes to promote proliferation, apoptosis, and morphogenesis in rat Schwann cells, in response to NRG1, TGF, and laminins, three major signals implicated in this patterning event. Strikingly,HuRalso binds to severalmRNAsencoding myelination-related proteins but, contrary to its typical function, negatively regulated their expression, likely to prevent ectopic myelination during development. These functions of HuR correlated with its abundance and subcellular localization, which were regulated by different signals in Schwann cells.
- Subjects
CARRIER proteins; ANTIGEN receptors; GENE expression; SCHWANN cells; MYELINATION; AXONS; CELL proliferation; APOPTOSIS
- Publication
Journal of Neuroscience, 2012, Vol 32, Issue 14, p4944
- ISSN
0270-6474
- Publication type
Article
- DOI
10.1523/JNEUROSCI.5868-11.2012