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- Title
Group IVA phospholipase A<sub>2</sub> is necessary for growth cone repulsion and collapse.
- Authors
Sanford, Staci D.; Yun, Bo Goen; Leslie, Christina C.; Murphy, Robert C.; Pfenninger, Karl H.
- Abstract
J. Neurochem. (2012) 120, 974-984. Abstract The repellent semaphorin 3A (Sema3A) causes growth cone turning or collapse by triggering cytoskeletal rearrangements and detachment of adhesion sites. Growth cone detachment is dependent on eicosanoid activation of protein kinase C epsilon (PKCε), but the characterization of the phospholipase A2 (PLA2) that releases arachidonic acid (AA) for eicosanoid synthesis has remained elusive. Here, we show, in rat dorsal root ganglion (DRG) neurons, that Sema3A stimulates PLA2 activity, that Sema3A-induced growth cone turning and collapse are dependent on the release of AA, and that the primary PLA2 involved is the group IV α isoform (GIVA). Silencing GIVA expression renders growth cones resistant to Sema3A-induced collapse, and GIVA inhibition reverses Sema3A-induced repulsion into attraction. These studies identify a novel, early step in Sema3A-signaling and a PLA2 necessary for growth cone repulsion and collapse.
- Subjects
AXONAL transport; PHOSPHOLIPASE A2; SEMAPHORINS; CYTOSKELETAL proteins; EICOSANOIDS; PROTEIN kinase C regulation; ARACHIDONIC acid
- Publication
Journal of Neurochemistry, 2012, Vol 120, Issue 6, p974
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2012.07651.x