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- Title
PACAP Stimulates the Release of the Secretogranin II-Derived Peptide EM66 from Chromaffin Cells.
- Authors
GUILLEMOT, JOHANN; AIT‐ALI, DJIDA; TURQUIER, VALERIE; MONTERO‐HADJADJE, MAITE; FOURNIER, ALAIN; VAUDRY, HUBERT; ANOUAR, YOUSSEF; YON, LAURENT
- Abstract
The aim of the present article was to examine the effect of PACAP on the release of the SgII-derived peptide EM66 from primary cultures of bovine chromaffin cells. PACAP dose dependently stimulated EM66 release from cultured chromaffin cells. A significant response was observed after 6 h of treatment with PACAP and increased to reach a 3.6-fold stimulation at 72 h. The stimulatory effect of PACAP was mediated through multiple signaling pathways, including calcium influx through L-type channels, PKA, PKC, and MAP-kinase cascades, to regulate EM66 release from chromaffin cells. These data suggest that EM66 may act downstream of the trans-synaptic stimulation of the adrenal medulla by neurocrine factors.
- Subjects
CHROMAFFIN cells; ADRENAL glands; SYMPATHETIC nervous system; CHROMOGRANINS; LIPOPROTEINS
- Publication
Annals of the New York Academy of Sciences, 2006, Vol 1070, Issue 1, p309
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1317.033