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- Title
Apoptosis in Cultured Cells Infected with Feline Calicivirus.
- Authors
ROBERTS, LISA O.; AL‐MOLAWI, NAEMA; CARTER, MICHAEL J.; KASS, GEORGE E. N.
- Abstract
Caliciviruses are important pathogens of man and animals; feline calicivirus (FCV) is responsible for an acute upper respiratory tract disease in cats. To date, little is known about the mechanism of cell damage induced by these viruses. We set out to determine if apoptosis played any role in cell death in FCV infection of cultured cells. We demonstrate that caspase-2, -3, and -7 were activated during FCV infection, as evidenced by pro-form processing and an increase in acetyl-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin cleavage activity, as well as cleavage of poly(ADP-ribose)polymerase. Caspase activation coincided with the condensation of chromatin. At about 8 h post infection we also detected cleavage of the FCV capsid protein; this was prevented by caspase inhibitors. Taken together these results suggest that FCV triggers apoptosis within infected cells and that caspases are involved in the cleavage of the capsid protein.
- Subjects
APOPTOSIS; PATHOGENIC microorganisms; NUCLEOPROTEINS; CHROMOSOMES; CELL nuclei; CELLULAR mechanics; CELL death; MICROORGANISMS; NUCLEIC acids
- Publication
Annals of the New York Academy of Sciences, 2004, Vol 1010, Issue 1, p587
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1299.110