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- Title
Development and Characterization of Polymorphic Microsatellite Markers for Sedum sarmentosum (Crassulaceae) and Their Cross-Species Transferability.
- Authors
Jing Xu; Fu-Yuan Hou; Ding-Rong Wan; Sha Wang; Dong-Mei Xu; Guang-Zhong Yang
- Abstract
Sedum sarmentosum is an important Chinese medicinal herb that exhibits anti-inflammatory, anti-angiogenic and anti-nociceptive properties. However, little is known about its genetic background. The first set of 14 microsatellite markers were isolated and characterized for S. sarmentosum using an SSR-enriched library. Fourteen polymorphic microsatellite markers were acquired with satisfactory amplifications and a polymorphic pattern in 48 S. sarmentosum individuals. The number of alleles ranged from 3 to 15. The observed and expected heterozygosities varied from 0.0833 to 0.8750 and 0.2168 to 0.9063, respectively. Two loci showed significant departure from the Hardy-Weinberg equilibrium. Cross-species amplification was carried out in other Sedum species. High rates of cross-species amplification were observed. The transferability value ranged from 85.7% in S. lineare to 64.3% in S. ellacombianum. These markers will be valuable for studying the genetic variation, population structure and germplasm characterization of S. sarmentosum and related Sedum species.
- Subjects
CRASSULACEAE; HERBAL medicine; MICROSATELLITE repeats; HARDY-Weinberg formula; AMPLIFICATION reactions; GENETIC markers
- Publication
Molecules, 2015, Vol 20, Issue 11, p19929
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules201119669