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- Title
A PCR-based microwell-plate hybrid capture assay for high-risk human papillomavirus.
- Authors
Wang, Yumei; Liu, Yan; Ding, Yaping; Sun, Nan; Gong, Yafang; Gao, Shangxian
- Abstract
Human papillomavirus (HPV) is associated with cervical cancer. In this study, we developed a high-throughput microwell-plate hybrid capture (MPHC) method for epidemiological studies of high-risk HPV (HRHPV). The results with 1238 cervical specimens from female outpatients showed a concordance rate of 94.3 % between the MPHC and Hybrid Capture II assay. The MPHC assay showed an average HRHPV rate of 29.3 % for high-risk populations in populous cities of China. The established MPHC assay could sensitively and specifically detect 13 types of HRHPV and is suitable for large-scale screening, especially in areas where real-time PCR or fluorescence equipment is unavailable.
- Subjects
POLYMERASE chain reaction; MICROBIOLOGICAL assay; PAPILLOMAVIRUSES; CERVICAL cancer research; EPIDEMIOLOGICAL research; BIOLOGICAL specimens
- Publication
Archives of Virology, 2014, Vol 159, Issue 12, p3365
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-014-2186-0