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- Title
Simple and rapid detection of swine hepatitis E virus by reverse transcription loop-mediated isothermal amplification.
- Authors
Zhang, Liang-Quan; Zhao, Fu-Rong; Liu, Zhi-Gang; Kong, Wei-Li; Wang, Heng; Ouyang, Yun; Liang, Huan-Bin; Zhang, Chao-Yi; Qi, Hai-Tao; Huang, Chang-Li; Guo, Si-Hu; Zhang, Gui-Hong
- Abstract
Hepatitis E virus (HEV) is an enteric pathogen of humans and animals, and pigs have been considered an important reservoir of this virus. Recent evidence has indicated the cross-species transmission of hepatitis E virus (HEV) from pigs to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/viscera. In this study, we have developed a one-step RT-LAMP assay for rapid detection of swine HEV. Specific primer sets targeting the ORF3 gene were designed. The sensitivity of the RT-LAMP assay was 10 copies/μl of RNA template, which was tenfold higher than that of RT-nPCR. The specificity of this assay was demonstrated by the lack of amplification of DNA/RNA from other swine viruses. Furthermore, a total of 41 bile samples were subjected to RT-LAMP and RT-nPCR. Eighteen positive samples were detected by RT-nPCR, while 36 positive samples were detected by RT-LAMP, indicating that the sensitivity of the RT-LAMP assay was higher than that of the conventional RT-nPCR assay. The RT-LAMP assay reported here may be used for diagnosis of swine HEV, not only in laboratories but also under field conditions.
- Subjects
HEPATITIS E virus; VIRUS identification; GENE amplification; REVERSE transcriptase polymerase chain reaction; ZOONOSES; DNA viruses; RNA viruses
- Publication
Archives of Virology, 2012, Vol 157, Issue 12, p2383
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-012-1425-5