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- Title
Differentiation of infectious bursal disease virus (IBDV) genome segment B of very virulent and classical lineage by RT-PCR amplification and restriction enzyme analysis.
- Authors
Islam, M.; Rahman, S.; Noor, M.; Chowdhury, E.; Müller, H.
- Abstract
Molecular characterization of IBDV usually relies on the analysis of segment A of the bi-segmented, double-stranded RNA genome. Although segments B of classical and very virulent IBDVs differ significantly, re-assortment of genome segments does occur, and molecular epidemiological studies demand the analysis of both segments. An RT-PCR and restriction enzyme analysis for molecular discrimination between genome segment B of classical and very virulent IBDVs is described. Tested on eight IBDV strains/isolates, the protocol successfully identified very virulent and classical IBDVs as well as a segment reassortant. This approach is a valuable tool for molecular epidemiological studies on IBDV.
- Subjects
REVERSE transcriptase polymerase chain reaction; VIRAL genomes; DNA restriction enzymes; EPIDEMIOLOGY; GENE amplification; MICROBIAL genomes; DEOXYRIBONUCLEASES
- Publication
Archives of Virology, 2012, Vol 157, Issue 2, p333
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-011-1159-9