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- Title
Analysis of the Specificity of Auto-Reactive Antibodies to Individual Fragments of the Extracellular Domain of Desmoglein 3 in Patients with Pemphigus Vulgaris.
- Authors
Kubanov, A. A.; Deryabin, D. G.; Shpilevaya, M. V.; Karamova, A. E.; Nikonorov, A. A.; Larina, E. N.; Aliev, T. K.; Dolgikh, D. A.; Bobik, T. V.; Smirnov, I. V.; Gabibov, A. G.; Kirpichnikov, M. P.
- Abstract
A method for the analysis of the epitope specificity of auto-reactive antibodies to desmoglein 3 (Dsg3) using competitive ELISA has been developed. It is based on a two-stage solid-phase ELISA with initial "depletion" of auto-reactive antibodies against the studied epitope and subsequent quantitative assessment of antibodies against full-length extracellular domain Dsg3. The proposed approach for assessing the specificity of the autoimmune response in patients with pemphigus vulgaris can provide in the future the possibility to personalize the therapy using plasmapheresis by preliminary selection of the antigenic composition of the extracorporeal immunosorbent.
- Subjects
PEMPHIGUS vulgaris; DESMOGLEINS; PLASMAPHERESIS; IMMUNOGLOBULINS; AUTOANTIBODIES
- Publication
Bulletin of Experimental Biology & Medicine, 2021, Vol 171, Issue 4, p475
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-021-05254-9