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- Title
Influence of inoculating forage with ferulate esterase producing lactic acid bacteria on ensilage and ruminal degradation of fiber.
- Authors
Nsereko, V. L.; Smiley, B. K.; Rutherford, W. M.; Spielbauer, A. .J.; Harman, E. K.; Harman, B. R.; Forrester, K. J.; Hettinger, G. H.
- Abstract
Ferulic acid (FA) cross-links restrict enzymatic degradation of cell wall polysaccharides in grasses (Graminaceae). By releasing FA from cell wall arabinoxylans, microbial ferulate esterases (FE) increase the susceptibility of plant cell walls to further enzymatic hydrolysis and increase the nutritive value of the forage. Because some lactic acid bacteria (LAB) produce FE, we investigated the effect of inoculating forage with FE producing LAB at ensiling on ruminal NDF degradation (NDFD). Among the 10000 LAB screened, approximately 500 produced FE; 8 of these were studied in more detail. The inoculants were Lactobacillus buchneri PTA6138 and NRRL B-30866; L. crispatus NRRL B-30868, 30869 and 30870; L. reuteri NRRL B-30867, L. brevis NRRL B-30865 and an unidentified Lactobacillus strain NRRLB-30871. Perennial ryegrass (Lolium perenne) was harvested and ensiled with or without (control) inoculation with each individual LAB, in triplicate laboratory silos. After a 30d fermentation period, silages were analyzed for fermentation characteristics, and incubated in situ (6mm) in Dacron bags (50µ pore size) for 48h in the rumens of 3 ruminally fistulated steers adapted to a diet of grass silage. Ruminal residues were composited across steers but within silo. Results were analyzed by oneway ANOVA, with treatment means compared by Dunnett's test. Compared with uninoculated silage, inoculating with L. buchneri strains PTA6138 and NRRL B-30866 increased silage pH and acetate (P<0.05) and reduced lactate concentration (P<0.05). Inoculation with NRRLB-30871 reduced silage pH (P<0.05), but other LAB had no significant effect on fermentation. With the exception of NRRLB-30871, all inoculants improved (P<0.05) NDFD by 5 to 7 units (9 to 11%). We conclude that inoculating forage with FE producing LAB at ensiling can improve ruminal NDFD. Because FE producing L. buchneri enhanced acetate concentrations, which increases aerobic stability, some FE producing LAB should enhance both the conservation and feeding value of ensiled crops.
- Subjects
LACTIC acid bacteria; SILAGE; FORAGE plants; PLANT cell walls; FORAGE; FERULIC acid; LOLIUM perenne
- Publication
Journal of Animal Science, 2006, Vol 84, p375
- ISSN
0021-8812
- Publication type
Article