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- Title
An evaluation of bacterial contamination of barriers used in periapical tissue regeneration: part 1 -- bacterial adherence.
- Authors
Sharma P; Mickel AK; Chogle S; Sharma PN; Han YW; Jones JJ
- Abstract
OBJECTIVE: To compare the adherence of Prevotella melaninogenica and Enterococcus faecalis to 3 guided tissue regeneration membranes: Atrisorb, Lambone, and OsseoQuest. It was hypothesized that OsseoQuest would show increased bacterial adherence compared to Lambone and Atrisorb. METHOD AND MATERIALS: The barriers were suspended in trypticase soy broth containing an inoculum of either P melaninogenica or E faecalis. The samples were incubated under appropriate conditions for 6, 24, and 48 hours. Following incubation, each membrane was mixed in fresh media in a vortex machine to dislodge adherent bacteria. The vortexed media was quantitatively assessed using serial dilutions for viable cell count. RESULTS: E faecalis exhibited higher adherence compared to P melaninogenica with time. Of the membranes tested, Lambone displayed the least bacterial adherence. CONCLUSION: An analysis of the results indicated that bacterial adherence was time-dependent for all membranes. Membrane structure, chemical configuration, hydrophobicity, and bacterial cell surface structure were suggested as factors contributing to variance in bacterial adherence.
- Publication
Quintessence International, 2008, Vol 39, Issue 2, p159
- ISSN
0033-6572
- Publication type
Journal Article