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- Title
Adenovirus Vaccine Containing Truncated SARS-CoV-2 Spike Protein S1 Subunit Leads to a Specific Immune Response in Mice.
- Authors
Chen, Keda; Shi, Danrong; Li, Chaonan; Fang, Zhongbiao; Guo, Yikai; Jiang, Wenjie; Li, Jiaxuan; Li, Hongyu; Yao, Hangping
- Abstract
The development of an efficient and safe coronavirus disease 2019 (COVID-19) vaccine is a crucial approach for managing the severe acute respiratory disease coronavirus 2 (SARS-CoV-2) pandemic in light of current conditions. In this study, we produced a shortened segment of the optimized SARS-CoV-2 spike gene (2043 bp, termed S1) that was able to encode a truncated S1 protein. The protein was tested to determine if it could elicit efficient immunization in mice against SARS-CoV-2. The presence of the S1 protein was confirmed with immunofluorescence and Western blotting. An adenovirus vaccine bearing the S1 gene fragment (Ad-S1) was administered intramuscularly to mice four times over 4 weeks. SARS-CoV-2 S1 protein humoral immunity was demonstrated in all immunized mice. The serum from immunized mice demonstrated excellent anti-infection activity in vitro. A robust humoral immune response against SARS-CoV-2 was observed in the mice after vaccination with Ad-S1, suggesting that the adenovirus vaccine may aid the development of vaccines against SARS-CoV-2 and other genetically distinct viruses.
- Subjects
COVID-19; SARS-CoV-2; IMMUNE response; HUMORAL immunity; ADENOVIRUSES
- Publication
Vaccines, 2023, Vol 11, Issue 2, p429
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines11020429