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- Title
Usp18 Expression in CD169+ Macrophages is Important for Strong Immune Response after Vaccination with VSV-EBOV.
- Authors
Friedrich, Sarah-Kim; Schmitz, Rosa; Bergerhausen, Michael; Lang, Judith; Cham, Lamin B.; Duhan, Vikas; Häussinger, Dieter; Hardt, Cornelia; Addo, Marylyn; Prinz, Marco; Asano, Kenichi; Lang, Philipp Alexander; Lang, Karl Sebastian
- Abstract
Ebola virus epidemics can be effectively limited by the VSV-EBOV vaccine (Ervebo) due to its rapid protection abilities; however, side effects prevent the broad use of VSV-EBOV as vaccine. Mechanisms explaining the efficient immune activation after single injection with the VSV-EBOV vaccine remain mainly unknown. Here, using the clinically available VSV-EBOV vaccine (Ervebo), we show that the cell-intrinsic expression of the interferon-inhibitor Usp18 in CD169+ macrophages is one important factor modulating the anti-Ebola virus immune response. The absence of Usp18 in CD169+ macrophages led to the reduced local replication of VSV-EBOV followed by a diminished innate as well as adaptive immune response. In line, CD169-Cre+/ki x Usp18fl/fl mice showed reduced innate and adaptive immune responses against the VSV wildtype strain and died quickly after infection, suggesting that a lack of Usp18 makes mice more susceptible to the side effects of the VSV vector. In conclusion, our study shows that Usp18 expression in CD169+ macrophages is one important surrogate marker for effective vaccination against VSV-EBOV, and probably other VSV-based vaccines also.
- Subjects
IMMUNE response; MACROPHAGES; VACCINATION; EBOLA virus; BIOMARKERS; BACTERIAL vaccines
- Publication
Vaccines, 2020, Vol 8, Issue 1, p142
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines8010142