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- Title
Isolation and characteristics of a thermophilic Bacillus strain, producer of inulinase.
- Authors
Gavrailov, Simeon; Ivanova, Viara
- Abstract
A strain of Bacillus sp. SG7, isolated from thermal water samples from the region of Velingrad, Bulgaria, showed good capacity to produce extracellular inulinase. The enzyme was synthesized in presence of monosaccharides and pure inulin, as carbon sources. With regard to the nitrogen source, the best results were obtained with yeast extract and other sources of proteic nitrogen, comparatively to the mineral nitrogen. Optimal growth temperature was 55-60∘C. No growth was established at 40∘C and 70∘C. It also showed rapid, good growth in wide pH range from 6.5 to 9.5 with optimum at pH 8.0-8.5. The optimum pH of the crude enzyme for inulin hydrolysis was found between 6.0 and 7.0 and the optimun temperature at 60∘C. The enzyme showed capacity to hydrolyse sucrose, raffinose and inulin from which it liberated only fructose units showing, therefore, an exo-action mechanism. Acting on inulins from several sources, the enzyme showed hydrolysis of the polissaccharide from chicory (Cichorium intibus), the inulins from dahlia (Dahlia pinnata) and Jerusalem artichoke (Helianthus tuberosus) roots.
- Subjects
BACILLUS (Bacteria); INULASE; CHEMICAL synthesis; ENZYMES
- Publication
Journal of BioScience & Biotechnology, 2014, p83
- ISSN
1314-6238
- Publication type
Article