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- Title
Propagation of Human Spermatogonial Stem Cells In Vitro.
- Authors
Sadri-Ardekani, Hooman; Mizrak, Sefika C.; van Daalen, Saskia K. M.; Korver, Cindy M.; Roepers-Gajadien, Hermien L.; Koruji, Morteza; Hovingh, Suzanne; de Reijke, Theo M.; de la Rosette, Jean J. M. C. H.; van der Veen, Fulco; de Rooij, Dirk G.; Repping, Sjoerd; van Pelt, Ans M. M.
- Abstract
The article offers information on a study which developed in vitro propagation of human spermatogonial stem cells from small testicular biopsies to derive an adequate number of cells for successful transplantation. Testis material donated by six adult men who undergone orchidectomy as part of prostate cancer treatment was utilized during the duration of the study from April 2007 to July 2009. To determine the presence of spermatogonia and spermatogonial markers, reverse transcriptase polymerase chain reaction and immunofluorescence were applied, respectively. Main outcome measure was the propagation of spermatogonial stem cells. Also presented in details are the research findings.
- Subjects
SPERMATOGENESIS; FERTILIZATION in vitro; STEM cells; TESTIS; PROSTATE cancer; REVERSE transcriptase polymerase chain reaction; IMMUNOFLUORESCENCE
- Publication
JAMA: Journal of the American Medical Association, 2009, Vol 302, Issue 19, p2127
- ISSN
0098-7484
- Publication type
Article
- DOI
10.1001/jama.2009.1689