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- Title
Assessment of SOX17 DNA methylation in cell free DNA from patients with operable gastric cancer. Association with prognostic variables and survival.
- Authors
Balgkouranidou, Ioanna; Karayiannakis, Anastasios; Matthaios, Dimitrios; Bolanaki, Heleni; Tripsianis, Gregorios; Apostolos Tentes, Antonios; Lianidou, Evi; Chatzaki, Ekaterini; Fiska, Aliki; Lambropoulou, Maria; Kolios, George; Kakolyris, Stylianos
- Abstract
Background: DNA methylation represents one of the most common epigenetic changes in human cancer providing important information regarding carcinogenesis. A possible role as a prognostic indicator has also been proposed. The aim of our study was to evaluate the prognostic significance of SOX17 promoter methylation status in patients with operable gastric cancer. Methods: Using methylation-specific PCR (MSP) we examined the incidence and prognostic significance of SOX17 methylation status in cell free circulating DNA in the serum of 73 patients with operable gastric cancer. Fifty-one patients were male (69.9%), their median age was 65 years, 43 patients (58.9%) had regional lymph node involvement and all had a Performance Status (WHO) of 0-1. Results: SOX17 promoter was found to be methylated in 43 out of 73 gastric cancer serum samples examined (58.9%). All 20 control serum samples from healthy individuals were negative. Overall survival (OS) was found to be significantly associated with SOX17 methylation (p=0.049). A significant correlation between methylation status and differentiation (p=0.031) was also observed. No other significant associations between different tumor parameters examined and SOX17 methylation status were observed. Conclusions: SOX17 promoter methylation in cell free DNA of patients with operable gastric cancer is a frequent event and may provide important information regarding prognosis in this group of patients.
- Subjects
DNA methylation; EPIGENETICS; CARCINOGENESIS; CANCER prognosis; STOMACH cancer
- Publication
Clinical Chemistry & Laboratory Medicine, 2013, Vol 51, Issue 7, p1505
- ISSN
1434-6621
- Publication type
Article
- DOI
10.1515/cclm-2012-0320