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- Title
Kan Kültürlerinden İzole Edilen Mikroorganizmaların Dağılımı ve Antimikrobiyal Duyarlılıkları.
- Authors
ŞAHİN, İdris; ÇALIŞKAN, Emel; ÖZTÜRK, Elif; YAVUZ, M. Tevfik; ALBAYRAK, Hilal Türkmen; KARADAĞ, Gülkan; ALTINÖZ AYTAR, Asiye
- Abstract
Purpose: In this study, the distribution of hospitalized patients and antimicrobial susceptibility of microorganisms isolated from blood culture were investigated. Methods: Blood samples sent to our laboratory by taking Bactec 9050 automated blood culture media bottles (Becton Dickinson, USA) were incubated at 35° C and under normal atmospheric conditions. İdentification of microorganisms was used conventional methods and / or the API identification systems (bioMérieux, Etoile, Fransa). Kirby-Bauer Disk diffusion method and / or was ATB STREP 5, ATB ENTEROC 5 (bioMérieux, Etoile, Fransa) performed for antibiotic susceptibility testing according the criteria of Clinical and Laboratory Standards Institute. Extended-spectrum beta-lactamase (ESBL) production was investigated with disk diffusion screening test in Gram-negative bacteria. Results: A total of 2,807 blood samples sent to our laboratory between July 2009 – 2010. 2,121 (75%) patients of didn’t reproductive, 583 (21%) of the cases were positive, 103 (4%) of the contamination was evaluated as an example. The most isolated pathogens, respectively, coagulasenegative staphylococci (CNS) (42.1%), Staphylococcus aureus (22%) and Escherichia coli (13%) were found. 54% KNS strains, 44 % S. aureus strains were resistant to methicillin. E. coli strains of extended-spectrum beta-lactamase (ESBL) prevalence of 14% and 21% of Klebsiella spp. strains were determined. İmipenem resistance in Pseudomonas aeruginosa strains were identified by 39%. The sensitivity of E. coli strains, 93% of amikacin, piperacillin-tazobactam 95%, the sensitivity of Klebsiella spp. strains, 100% of amikacin, piperacillin-tazobactam 93% were determined. The results of the study were examined by comparing with similar studies. Conclusion: As a result, the distribution of bacteria isolated from blood cultures and antimicrobial susceptibility patterns vary among centers, each center, therefore, thought to be useful in their assessment of the results at regular intervals.
- Publication
Duzce Medical Journal, 2013, Vol 15, Issue 2, p11
- ISSN
1307-671X
- Publication type
Article