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- Title
Effects of TNF- α blocking on experimental periodontitis and type 2 diabetes in obese diabetic Zucker rats.
- Authors
Grauballe, Morten Bay; Østergaard, Jakob Appel; Schou, Søren; Flyvbjerg, Allan; Holmstrup, Palle
- Abstract
Objective Tumour necrosis factor α ( TNF- α) is considered a key signalling modulator in the pathogenesis of both periodontitis ( PD) and type 2 diabetes mellitus ( DM2). This study aims at elucidating the effect of TNF- α blocking on the interplay between PD and DM2. Methods Obese diabetic Zucker rats and their lean littermates were divided into five treatment groups with or without periodontitis. Anti- TNF- α treatment was provided with Etanercept injections. Diabetic state was evaluated by oral glucose tolerance test, the homeostatic model assessment, free fatty acids and blood glucose. Systemic inflammation was assessed by measurement of interleukin ( IL)-1 β, IL-6 and TNF- α in plasma. Kidney complications were evaluated by real-time rt PCR, creatinine clearance rate, urinary albumin excretion and increase in weight. PD was evaluated by registration of alveolar bone level. Results After 4 weeks the diabetic state was modified by Etanercept treatment with lower insulin levels and lower homeostatic model assessment. Furthermore, while kidney complications were reduced by Etanercept treatment, PD had no effect. PD was influenced by diabetic state, but the impact was attenuated by Etanercept treatment. Conclusion In this study anti- TNF- α treatment improved glucose tolerance and compensated for the increased periodontal disease in obese diabetic Zucker. PD did not influence diabetic parameters assessed including complications of the rats kidneys.
- Subjects
DENMARK; ACADEMIC medical centers; ANALYSIS of variance; ANIMAL experimentation; BIOLOGICAL models; BLOOD testing; INTERLEUKINS; TYPE 2 diabetes; OBESITY; PERIODONTITIS; POLYMERASE chain reaction; RATS; RESEARCH funding; STATISTICS; DATA analysis; DATA analysis software; KRUSKAL-Wallis Test; CHEMICAL inhibitors
- Publication
Journal of Clinical Periodontology, 2015, Vol 42, Issue 9, p807
- ISSN
0303-6979
- Publication type
Article
- DOI
10.1111/jcpe.12442