We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Licensing of DNA replication by a multi-protein complex of MCM/P1 proteins in Xenopus eggs.
- Authors
Kubota, Yumiko; Mimura, Satoru; Nishimoto, Shin-ichi; Masuda, Taro; Nojima, Hiroshi; Takisawa, Haruhiko
- Abstract
In eukaryotes, chromosomal DNA is licensed for a single round of replication in each cell cycle. Xenopus MCM3 protein has been implicated in the licensing of replication in egg extract. We have cloned cDNAs encoding five immunologically distinct proteins associated with Xenopus MCM3 as members of the MCM/P1 family. Six Xenopus MCM proteins formed a physical complex in the egg extract, bound to unreplicated chromatin before the formation of nuclei, and apparently displaced from replicated chromatin. The requirement of six XMCM proteins for the replication activity of the egg extract before nuclear formation suggests that their re-association with replicated chromatin at the end of the mitotic cell cycle is a key step for the licensing of replication.
- Subjects
DNA replication; EUKARYOTIC cells; CELL cycle; PROTEINS; CHROMATIN; CHROMOSOMES
- Publication
EMBO Journal, 1997, Vol 16, Issue 11, p3320
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/16.11.3320