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- Title
Exploring the Concept of In Vivo Guided Tissue Engineering by a Single-Stage Surgical Procedure in a Rodent Model.
- Authors
Chamorro, Clara Ibel; Zeiai, Said; Juul, Nikolai; Willacy, Oliver; Huo, Jinxing; Hilborn, Jöns; Fossum, Magdalena
- Abstract
In severe malformations with a lack of native tissues, treatment options are limited. We aimed at expanding tissue in vivo using the body as a bioreactor and developing a sustainable single-staged procedure for autologous tissue reconstruction in malformation surgery. Autologous micro-epithelium from skin was integrated with plastically compressed collagen and a degradable knitted fabric mesh. Sixty-three scaffolds were implanted in nine rats for histological and mechanical analyses, up to 4 weeks after transplantation. Tissue integration, cell expansion, proliferation, inflammation, strength, and elasticity were evaluated over time in vivo and validated in vitro in a bladder wound healing model. After 5 days in vivo, we observed keratinocyte proliferation on top of the transplant, remodeling of the collagen, and neovascularization within the transplant. At 4 weeks, all transplants were fully integrated with the surrounding tissue. Tensile strength and elasticity were retained during the whole study period. In the in vitro models, a multilayered epithelium covered the defect after 4 weeks. Autologous micro-epithelial transplants allowed for cell expansion and reorganization in vivo without conventional pre-operative in vitro cell propagation. The method was easy to perform and did not require handling outside the operating theater.
- Subjects
OPERATIVE surgery; TISSUE engineering; AUTOGRAFTS; TISSUES; TENSILE strength; RODENTS; HEALING
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 20, p12703
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms232012703