We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Elicitation of Diosgenin Production in Trigonella foenum-graecum (Fenugreek) Seedlings by Methyl Jasmonate.
- Authors
Chaudhary, Spandan; Chikara, Surendra K.; Sharma, Mahesh C.; Chaudhary, Abhinav; Syed, Bakhtiyar Alam; Chaudhary, Pooja S.; Mehta, Aditya; Patel, Maulik; Ghosh, Arpita; Iriti, Marcello
- Abstract
The effects of methyl jasmonate (MeJA), an elicitor of plant defense mechanisms, on the biosynthesis of diosgenin, a steroidal saponin, were investigated in six fenugreek (Trigonella foenum-graecum) varieties (Gujarat Methi-2, Kasuri-1, Kasuri-2, Pusa Early Branching, Rajasthan Methi and Maharashtra Methi-5). Treatment with 0.01% MeJA increased diosgenin levels, in 12 days old seedlings, from 0.5%-0.9% to 1.1%-1.8%. In addition, MeJA upregulated the expression of two pivotal genes of the mevalonate pathway, the metabolic route leading to diosgenin: 3-hydroxy-3-methylglutaryl-CoA reductase (HMG) and sterol-3-β-glucosyl transferase (STRL). In particular, MeJA increased the expression of HMG and STRL genes by 3.2- and 22.2-fold, respectively, in the Gujarat Methi-2 variety, and by 25.4- and 28.4-fold, respectively, in the Kasuri-2 variety. Therefore, MeJA may be considered a promising elicitor for diosgenin production by fenugreek plants.
- Subjects
JASMONATE; FENUGREEK; PLANT defenses; STEROID saponins; DIOSGENIN
- Publication
International Journal of Molecular Sciences, 2015, Vol 16, Issue 12, p29889
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms161226208