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- Title
Effect of Qingxin Kaiqiao Fang on Hippocampus mRNA Expression of the Inflammation-Related Genes IL-1β, GFAP, and Aβ in an Alzheimer’s Disease Rat Model.
- Authors
Mao, Dan-Dan; Yang, Wen-Yu; Li, Yan; Lin, Jian-Wei; Gao, Shi-Yu; Wang, Yi-Ru; Hu, Hai-Yan
- Abstract
<italic>Objective</italic>. To investigate the effects of QKF on expression of amyloid-beta (A<italic>β</italic>), interleukin-1 beta (IL-1<italic>β</italic>), and glial fibrillary acidic protein (GFAP) using a rat model of AD.<italic> Materials and Methods</italic>. Fifty-six male Sprague-Dawley rats were randomly divided into seven groups (eight rats each): control group, sham-operated group, AD model group, groups of AD rats administered with low, medium, and high doses of QKF, and the donepezil group. AD was established by bilateral injection of <italic>β</italic>-amyloid (A<italic>β</italic>) 1–40 into the hippocampus. Two days after AD was established, drugs were administered by gavage. After 14 days of treatment, we used RT-PCR, Western blotting, and immunohistochemistry to measure the transcript expression and protein abundance of A<italic>β</italic>, IL-1<italic>β</italic>, and GFAP, and methenamine silver staining was used to detect amyloid protein particle deposition.<italic> Results</italic>. Compared to the control group, the rats from the AD model group showed significantly greater expression levels of A<italic>β</italic>, IL-1<italic>β</italic>, and GFAP. However, these differences in expression were abolished by treatment with QKF or donepezil.<italic> Conclusion</italic>. QKF possesses therapeutic potential against AD because it downregulated A<italic>β</italic>, IL-1<italic>β</italic>, and GFAP in the hippocampus of AD rats. Future studies should further examine the mechanisms through which QKF produces its effects and the consequences of long-term QKF administration.
- Subjects
ALZHEIMER'S disease; ANIMAL experimentation; BIOLOGICAL models; GENE expression; HERBAL medicine; HIPPOCAMPUS (Brain); IMMUNOHISTOCHEMISTRY; INTERLEUKINS; CHINESE medicine; POLYMERASE chain reaction; PROTEINS; RATS; RNA; STATISTICAL sampling; SILVER staining (Microscopy); WESTERN immunoblotting; REVERSE transcriptase polymerase chain reaction; DONEPEZIL
- Publication
Evidence-based Complementary & Alternative Medicine (eCAM), 2018, Vol 2018, p1
- ISSN
1741-427X
- Publication type
Article
- DOI
10.1155/2018/9267653