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- Title
Adriamycin induces H2AX phosphorylation in human spermatozoa.
- Authors
Li, Zhong-Xiang; Wang, Ting-Ting; Wu, Yan-Ting; Xu, Chen-Ming; Dong, Min-Yue; Sheng, Jian-Zhong; Huang, He-Feng
- Abstract
Aim: To investigate whether adriamycin induces DNA damage and the formation of γH2AX (the phosphorylated form of histone H2AX) foci in mature spermatozoa. Methods: Human spermatozoa were treated with adriamycin at different concentrations. γH2AX was analyzed by immunofluorescent staining and flow cytometry and double-strand breaks (DSB) were detected by the comet assay. Results: The neutral comet assay revealed that the treatment with adriamycin at 2 μg/mL for different times (0.5, 2, 8 and 24 h), or for 8 h at different concentrations (0.4, 2 and 10 μg/mL), induced significant DSB in spermatozoa. Immunofluorent staining and flow cytometry showed that the expression of γH2AX was increased in a dose-dependent and time-dependant manner after the treatment of adriamycin. Adriamycin also induced the concurrent appearance of DNA maintenance/repair proteins RAD50 and 53BP1 with γH2AX in spermatozoa. Wortmannin, an inhibitor of the phosphatidylinositol 3-kinase (PI3K) family, abolished the co-appearance of these two proteins with γH2AX. Conclusion: Human mature spermatozoa have the same response to DSB-induced H2AX phosphorylation and subsequent recruitment of DNA maintenance/ repair proteins as somatic cells.
- Publication
Asian Journal of Andrology, 2008, Vol 10, Issue 5, p749
- ISSN
1008-682X
- Publication type
Article
- DOI
10.1111/j.1745-7262.2008.00400.x