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- Title
The utility of DNA methylation analysis in elderly patients with pilocytic astrocytoma morphology.
- Authors
Suruga, Yasuki; Satomi, Kaishi; Otani, Yoshihiro; Fujii, Kentaro; Ishida, Joji; Uneda, Atsuhito; Tsuboi, Nobushige; Makino, Keigo; Hirano, Shuichiro; Kemmotsu, Naoya; Imoto, Ryoji; Mizuta, Ryo; Tomita, Yusuke; Yasuhara, Takao; Washio, Kana; Yanai, Hiroyuki; Matsushita, Yuko; Hibiya, Yuko; Yoshida, Akihiko; Capper, David
- Abstract
Purpose: Pilocytic astrocytoma (PA) is a circumscribed low-grade astrocytic glioma, generally considered to be associated with a good prognosis. However, a subset of PA patients shows unfavorable outcomes. In this study, we retrospectively reviewed PA patients and performed further molecular analysis, such as DNA methylation profiling, to identify prognostic factors. Methods: We analyzed 29 histologically-confirmed PA patients from a single center from 2002 to 2021 and conducted integrated molecular analyses among elderly PA patients since age was an independent prognostic factor for poor outcomes. Results: The median age at diagnosis was 14 years (range 3–82 years) and 4 patients (14%) were elderly (patients ≥ 60 years old). Age over 60 was associated with poor progression-free survival and overall survival. We performed DNA methylation analysis on 2 of the 4 elderly patients. Both cases were histologically diagnosed as PA, but DNA methylation profiling revealed one as high-grade astrocytoma with piloid features (all methylation class scores were below 0.3 in both v11b4 and v12.5) and the other as glioblastoma, IDH-wildtype (score was over 0.5 in both v11b4 and v12.5), using the German Cancer Research Center methylation profiling classifiers and t-SNE analysis. Conclusions: Elderly patients with PA morphology showed unfavorable outcomes in this cohort. In those patients, further molecular analysis and DNA methylation profiling revealed the possibility of high-grade astrocytic tumors, including newly defined entities.
- Subjects
OLDER patients; DNA methylation; DNA analysis; ASTROCYTOMAS; MORPHOLOGY; TUMOR budding
- Publication
Journal of Neuro-Oncology, 2022, Vol 160, Issue 1, p179
- ISSN
0167-594X
- Publication type
Article
- DOI
10.1007/s11060-022-04131-3