We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Cyclic Tensile Stretch Stimulates the Release of Reactive Oxygen Species from Osteoblast-like Cells.
- Authors
Yamamoto, N.; Fukuda, K.; Matsushita, T.; Matsukawa, M.; Hara, F.; Hamanishi, C.
- Abstract
It is known that the excessive generation of reactive oxygen species (ROS) is a significant factor in tissue injury observed in many disease states. To determine whether extreme levels of mechanical stress applied to osteoblasts enhances ROS synthesis, we loaded cyclic tensile stretch on osteoblast-like HT-3 cells. Cyclic tensile stretch loaded on these cells clearly enhanced ROS synthesis in a time- and magnitude-dependent fashion. Cyclic tensile stretch also enhanced superoxide dismutase (SOD) activity. The disruption of microfilaments with cytochalasin D abolished the stress-induced ROS synthesis. Rotenone, an inhibitor of the mitochondrial electron transport chain, enhanced stress-induced ROS synthesis. These data suggest that actin filament and mitochondria are involved in this action.
- Subjects
REACTIVE oxygen species; CELLS; BONE diseases; ACTIN; MITOCHONDRIA
- Publication
Calcified Tissue International, 2005, Vol 76, Issue 6, p433
- ISSN
0171-967X
- Publication type
Article
- DOI
10.1007/s00223-004-1188-4