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- Title
Human adult germline stem cells in question.
- Authors
Ko, Kinarm; Araúzo-Bravo, Marcos J.; Tapia, Natalia; Kim, Julee; Qiong Lin; Bernemann, Christof; Han, Dong Wook; Gentile, Luca; Reinhardt, Peter; Greber, Boris; Schneider, Rebekka K.; Kliesch, Sabine; Zenke, Martin; Schöler, Hans R.
- Abstract
Arising from: S. Conrad et al. 456, 344–349 (2008); Conrad et al. replyConrad et al. have generated human adult germline stem cells (haGSCs) from human testicular tissue, which they claim have similar pluripotent properties to human embryonic stem cells (hESCs). Here we investigate the pluripotency of haGSCs by using global gene-expression analysis based on their gene array data and comparing the expression of pluripotency marker genes in haGSCs and hESCs, and in haGSCs and human fibroblast samples derived from different laboratories, including our own. We find that haGSCs and fibroblasts have a similar gene-expression profile, but that haGSCs and hESCs do not. The pluripotency of Conrad and colleagues’ haGSCs is therefore called into question.
- Subjects
GERM cells; EMBRYONIC stem cell research; STEM cell research; EMBRYOLOGY; HUMAN cloning; FIBROBLASTS; GENE expression; GENETIC regulation; MOLECULAR genetics
- Publication
Nature, 2010, Vol 465, Issue 7301, pE1
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/nature09089