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- Title
Detection of Fœtal and Sickle-cell Hæmoglobins in Human Anæmias.
- Authors
BEAVEN, G. H.; WHITE, J. C.
- Abstract
WE have systematically examined a large number (175) of normal and anæmic bloods for the presence of fœtal hæmoglobin (F-Hb)1 by three methods: (a) the position of the tryptophan fine-structure band2, using the moving-plate method of Holiday3, from which the relative proportions of adult (A-Hb)1 and fœtal hæmoglobin can be estimated4 when more than 10 per cent of the latter is present, though smaller proportions cannot be detected; (b) the Rich 'slope' method5, using the Unicam SP.500 spectrophotometer, which has the same order of sensitivity; (c) the rate of alkaline denaturation at pH 12.8, which provides a sensitive means of detecting the more alkaline-resistant fœtal hæmoglobin6.
- Publication
Nature, 1953, Vol 172, Issue 4387, p1006
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/1721006a0