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- Title
Development of a Bispecific Monoclonal Antibody to Pesticide Carbofuran and Triazophos Using Hybrid Hybridomas.
- Authors
JIN, R. Y.; GUO, Y. R.; WANG, C. M.; WU, J. X.; ZHU, G. N.
- Abstract
A mouse hybrid hybridoma (tetradoma) was derived from fusing hybridomas producing monoclonal antibody to N-methylcarbamate pesticide carbofuran with hybridomas producing monoclonal antibody to organophosphorus pesticide Triazophos. The prepared tetradoma line (12C1 to 2H12) secreted hybrid immunoglobulin exhibiting parental and bispecific binding characteristics. The effect of relevant physicochemical factors on the immunoassay based on the 12C1 to 2H12 bispecific monoclonal antibody had been studied to optimize the ELISA performance. The developed immunoassay showed that the detection limit (I20) were 0.36 and 1.89 ng/mL for triazophos and carbofuran, respectively, without obvious cross-reactivity to other related compounds. Water samples spiked with triazophos at 0.5, 1, and 5 ng/mL or carbofuran at 5, 10, and 20 ng/mL were directly analyzed by the developed ELISA format. The mean recovery of triazophos and carbofuran were 108.1% and 107.5%, with variation coefficient of 15.9% and 17.7%, respectively.
- Subjects
MICE; HYBRIDOMAS; MONOCLONAL antibodies; PESTICIDES; ORGANOPHOSPHORUS compounds; CARBOFURAN
- Publication
Journal of Food Science (Wiley-Blackwell), 2009, Vol 74, Issue 1, pT1
- ISSN
0022-1147
- Publication type
Article
- DOI
10.1111/j.1750-3841.2008.01002.x