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- Title
ORC1 interacts with c-Myc to inhibit E-box-dependent transcription by abrogating c-Myc–SNF5/INI1 interaction.
- Authors
Takayama, Masa-aki; Taira, Takahiro; Tamai, Katsuyuki; Iguchi-Ariga, Sanae M. M.; Ariga, Hiroyoshi
- Abstract
Background The c-myc oncogene product (c-Myc) is a transcription factor that forms a complex with Max and recognizes the E-box sequence. c-Myc plays key functions in cell proliferation, differentiation and apoptosis. As for its activity towards cell proliferation, it is generally thought that c-Myc transactivates the E-box-containing genes that encode proteins essential to cell-cycle progression. Despite the characterization of candidate genes regulated by c-Myc in culture cells, these have still not been firmly recognized as real target genes for c-Myc. Results We found that c-Myc directly bound to the N-terminal region of origin recognition complex-1 (ORC1), a region that is responsible for gene silencing, in a state of complex containing other ORC subunits and Max in vivo and in vitro. Furthermore, ORC1 inhibited E-box-dependent transcription activity of c-Myc by competitive binding to the C-terminal region of c-Myc with SNF5, a component of chromatin remodelling complex SNF/Swi1. Conclusions These results suggest that ORC1 suppresses the transcription activity of c-Myc by its recruitment into an inactive form of chromatin during some stage of the cell cycle.
- Subjects
GENETIC transcription; ONCOGENES
- Publication
Genes to Cells, 2000, Vol 5, Issue 6, p481
- ISSN
1356-9597
- Publication type
Article
- DOI
10.1046/j.1365-2443.2000.00338.x