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- Title
Sodium butyrate modulates cell cycle-related proteins in HT29 human colonic adenocarcinoma cells.
- Authors
Coradini, D.; Pellizzaro, C.; Marimpietri, D.; Abolafio, G.; Daidone, M. G.
- Abstract
Sodium butyrate (NaB), a product of colonic fermentation of dietary fibre, has been shown to inhibit cell proliferation by blocking cells in the G0 /G1 phase of the cell cycle through a mechanism of action still not completely understood. We investigated the effect of NaB on the level of some G1 phase-related proteins in a colon carcinoma cell line (HT29). In particular, we addressed our attention to cyclin D1 (the key regulator of G1 S progression), p21waf1/cip1 (the main inactivator of the cyclin D/cdk complex), and p53 (the most important regulator of p21waf1/cip1 gene transcription). At inhibitory concentrations (higher than 1 mM) NaB reduced cyclin D1 and p53 level in a dose-dependent manner and sustained the synthesis of p21waf1/cip1 , probably in a p53-independent way, accounting for the G0 /G1 block observed by flow cytometry. Present results provide further evidence on the molecular mechanism at the basis of the physiological role of NaB and support the hypothesis that an unbalanced diet, poor in carbohydrates and therefore in NaB, could result in functional alterations with clinical and carcinogenic implications.
- Subjects
COLON cancer; GROWTH factors
- Publication
Cell Proliferation, 2000, Vol 33, Issue 3, p139
- ISSN
0960-7722
- Publication type
Article
- DOI
10.1046/j.1365-2184.2000.00173.x