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- Title
Light-Induced Protein Dimerization by One- and Two-Photon Activation of Gibberellic Acid Derivatives in Living Cells.
- Authors
Schelkle, Korwin M.; Griesbaum, Tristan; Ollech, Dirk; Becht, Steffy; Buckup, Tiago; Hamburger, Manuel; Wombacher, Richard
- Abstract
We developed a highly efficient system for light-induced protein dimerization in live cells using photo-caged derivatives of the phytohormone gibberellic acid (GA3). We demonstrate the application of the photo-activatable chemical inducer of dimerization (CID) for the control of protein translocation with high spatiotemporal precision using light as an external trigger. Furthermore, we present a new two-photon (2P)-sensitive caging group, whose exceptionally high two-photon cross section allows the use of infrared light to efficiently unleash the active GA3 for inducing protein dimerization in living cells.
- Subjects
DIMERIZATION; NUCLEAR activation analysis; GIBBERELLIC acid; PLANT hormones; INFRARED radiation
- Publication
Angewandte Chemie International Edition, 2015, Vol 54, Issue 9, p2825
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201409196