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- Title
MALDI Mass Spectrometry Imaging of 1-Methyl-4-phenylpyridinium (MPP) in Mouse Brain.
- Authors
Kadar, Hanane; Le Douaron, Gael; Amar, Majid; Ferrié, Laurent; Figadère, Bruno; Touboul, David; Brunelle, Alain; Raisman-Vozari, Rita
- Abstract
Parkinson's disease (PD) is the second most common neurodegenerative disorder affecting ~1 % of the population older than 60 years. The administration of the proneurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in mice is one of the most widely used approach to elucidate the mechanisms of cell death involved in PD. Its toxicity is attributed to its active metabolite 1-methyl-4-phenylpyridinium (MPP). However, the magnitude of the PD-like neurodegeneration induced by MPTP depends on many variables, including the route of administration. Different groups, including us, demonstrated that intranasal (i.n.) administration of MPTP constitutes a new route of toxin delivery to the brain that mimics environmental exposure to neurotoxins. In particular, our previous data showed that mice submitted to acute i.n. MPTP administration displayed a significant decrease of striatal dopamine (DA) and a loss of dopaminergic (DA) neurons in the substantia nigra pars compacta. However, little is known about the timing and the anatomical distribution of MPP after i.n. MPTP administration in mice. In the present study, C57BL/6J mice received one dose of i.n. MPTP (1 mg/nostril) and were sacrificed at two different times after the administration. Using matrix-assisted laser desorption-ionization mass spectrometry imaging, a new technique for the detection of endogenous unlabeled molecules in tissue sections, we showed for the first time the MPP anatomical distribution in different brain regions. We demonstrated that the toxin first reached almost all the brain areas; however, in a second time MPP remained highly concentrated in the olfactory bulb, the basal ganglia, the ventral mesencephalon, and the locus coeruleus, regions differently affected in PD.
- Subjects
MATRIX-assisted laser desorption-ionization; MASS spectrometry; LABORATORY mice; PARKINSON'S disease; NEURODEGENERATION; DRUG administration
- Publication
Neurotoxicity Research, 2014, Vol 25, Issue 1, p135
- ISSN
1029-8428
- Publication type
Article
- DOI
10.1007/s12640-013-9449-5