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- Title
Expression of Interleukin-6, Interleukin-6 Receptor (gp80), and the Receptor’s Signal-Transducing Subunit (gp130) in Human Normal Pituitary Glands and Pituitary Adenomas.
- Authors
Kurotani, Reiko; Yasuda, Masanori; Oyama, Kenichi; Egashira, Noboru; Sugaya, Muthumi; Teramoto, Akira; Osamura, R. Yoshiyuki
- Abstract
Interleukin-6 (IL-6) is an important cytokine in cell proliferation and differentiation in several organs. It has also been reported that IL-6 plays a role in secretion or release of pituitary hormones in pituitary hormone-secreting cells and pituitary adenomas, but convincing data in situ have not yet been reported. In this study, we examined the participation of IL-6 in the production of pituitary hormones and the differences between human normal pituitary glands and pituitary adenomas by determination of the localization or expression of IL-6, IL-6 receptor (IL-6R, gp80), and the signal-transducing subunit (gp130) of the receptor using immunohistochemical staining and RT-PCR. IL-6 was mainly expressed in ACTH- and FSH/LH-secreting cells in normal pituitary glands, as shown by double staining. gp80 and gp130 were coexpressed in almost all GH- and PRL-secreting cells and in approximately 30% of FSH/LH-secreting cells. RT-PCR showed that IL-6 mRNA was expressed in only one of all the pituitary adenomas examined, whereas gp80 and gp130 mRNAs were detected in all these pituitary adenomas. In conclusion, IL-6 was mainly expressed in ACTH- and FSH/LH-secreting cells, and the receptors were expressed in GH-, PRL- and FSH/ LH-secreting cells in human normal pituitary glands. Furthermore, our data emphasized that the mechanism of IL-6 function in human pituitary adenoma cells is distinct from that in normal pituitary cells.
- Subjects
CYTOKINES; PITUITARY hormones; PITUITARY gland; ADENOMA; IMMUNOHISTOCHEMISTRY; CELLS
- Publication
Modern Pathology, 2001, Vol 14, Issue 8, p791
- ISSN
0893-3952
- Publication type
Article
- DOI
10.1038/modpathol.3880392