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- Title
Expression and Biochemical Characterization of β-Primeverosidase and Application of β-Primeverosylamidine to Affinity Purification.
- Authors
Saino, Hiromichi; Mizutani, Masaharu; Hiratake, Jun; Sakata, Kanzo
- Abstract
The article reports on results of a study of the expression and biochemical characterization of beta-primeverosidase and application of beta-primeresoylamidine to affinity purification. A description of the experimental set-up and measurement methods is presented. The study concluded that beta-primeverosylamidine is an ideal chemical tool for probing disaccharide recognition in the active site of beta-primeverosidase.
- Subjects
BIOCHEMISTRY; CHEMISTRY; GLYCOSIDASES; HYDROLYSIS; DISACCHARIDES
- Publication
Bioscience, Biotechnology & Biochemistry, 2008, Vol 72, Issue 2, p376
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1271/bbb.70447