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- Title
Simple and objective method for routine detection of the macular pigment xanthophyll.
- Authors
Dietrich Schweitzer; Susanne Jentsch; Jens Dawczynski; Martin Hammer; Ute E.K. Wolf-Schnurrbusch; Sebastian Wolf
- Abstract
A new simple method for two-dimensional determination of optical density of macular pigment xanthophyll (ODx) in clinical routine is based on a single blue-reflection fundus image. Individual different vignetting is corrected by a shading function. For its construction, nodes are automatically found in structureless image regions. The influence of stray light in elderly crystalline lenses is compensated by a correction function that depends on age. The reproducibility of parameters in a one-wavelength reflection method determined for three subjects (47, 61, and 78 years old) was: maxODx 6.3, meanODx 4.6, volume 6, and area 6 already before stray-light correction. ODx was comparable in pseudophakic and in an eye with a crystalline lens of the same 11 subjects after stray-light correction. Significant correlation in ODx was found between the one-wavelength reflection method and the two-wavelength autofluorescence method for pseudophakic and cataract eyes of 19 patients suffering from dry age-related macular degeneration (AMD) (R2 0.855). In pseudophakic eyes, maxODx was significantly lower for dry AMD (n 45) (ODx 0.491±0.102 ODU) than in eyes with healthy fundus (n 22) (ODx 0.615±0.103 ODU) (p 0.000033). Also in eyes with crystalline lens, maxODx was lower in AMD (n 125) (ODx 0.610±0.093 ODU) than in healthy subjects (n 45) (ODx 0.674±0.098 ODU) (p 0.00019). No dependence on age was found in the pseudophakic eyes both of healthy subjects and AMD patients.
- Subjects
RETINAL degeneration; XANTHOPHYLLS; OPACITY (Optics); OPTICAL detectors; RADIATIVE corrections; IMAGE analysis; FLUORESCENCE; OPTICAL reflection
- Publication
Journal of Biomedical Optics, 2010, Vol 15, Issue 6, p061714
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.3526358