We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Gene expression profiles of disc tissues and peripheral blood mononuclear cells from patients with degenerative discs.
- Authors
Yin-gang Zhang; Xiong Guo; Zhengming Sun; Guanghui Jia; Peng Xu; Shijie Wang; Zhang, Yin-gang; Guo, Xiong; Sun, Zhengming; Jia, Guanghui; Xu, Peng; Wang, Shijie
- Abstract
The objective of this study was to analyze gene expression profiles of intervertebral disc samples and peripheral blood mononuclear cells (PBMCs) from patients with degenerative discs using Agilent's Human 1A Oligo microarray. RNA samples from disc tissue and PBMCs were obtained from patients with degenerative discs and from subjects in a control group. RNA samples were reverse-transcribed into Cy5-labeled cRNA, combined with a Cy3-labeled reference and hybridized to oligonucleotide microarrays. Microarrays were scanned by Gene-Pix 4000B and data were analyzed using GenePixPro 3.0 software. The microarray data were validated in the same RNA samples by qRT-PCR analysis of selected genes. For the disc tissue, the mRNA expressions of 522 genes changed obviously in the degeneration group, accounting for approximately 2.64% of all analyzed transcripts. These included transcription-related, ion channel and transport protein, receptor, protein synthesis and modifying, growth factor, etc. For PBMCs, the expressions of 62 genes changed obviously in the patients in the degeneration group. These changes included ion channel, transport protein, transcription-related, DNA synthesis and repair, metalloprotease, immune globulin-related, growth factor-related, extracellular matrix-related, adhesion molecule, etc. Analyzed on the association of the differential expression of genes between disc tissue and PBMCs, some genes were not compatible. The course of intervertebral disc denegation is a complicated dynamic process, however, and may mainly be local pathogenesis. These findings furnish new data for the mechanistic investigation of degenerative discs.
- Subjects
GENE expression; INTERVERTEBRAL disk; PROTEIN synthesis; CYTOKINES; MONONUCLEOSIS; PROTEIN microarrays; RNA metabolism; COMPARATIVE studies; GENES; IMMUNITY; INTERVERTEBRAL disk displacement; SPINE diseases; RESEARCH methodology; MEDICAL cooperation; NURSE anesthetists; POLYMERASE chain reaction; RESEARCH; EVALUATION research; REVERSE transcriptase polymerase chain reaction; OLIGONUCLEOTIDE arrays; GENE expression profiling; MONONUCLEAR leukocytes; NUCLEIC acid amplification techniques
- Publication
Journal of Bone & Mineral Metabolism, 2010, Vol 28, Issue 2, p209
- ISSN
0914-8779
- Publication type
journal article
- DOI
10.1007/s00774-009-0120-4