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- Title
Epinephrine Affects Hemodynamics of Noninnervated Normal and All-Trans Retinoic Acid-Treated Embryonic Chick Hearts.
- Authors
Kroese, Jeanne Margot; Broekhuizen, Monique L. A.; Poelmann, Rob E.; Mulder, Paul G. H.; Wladimiroff, Juriy W.
- Abstract
Objective: To study the acute effect of epinephrine on hemodynamics of noninnervated normal and retinoicacid- treated embryos. Design: Prospective interventional study design. Methods: A total of 190 stage 15 (50-55 h of incubation) chick embryos were randomly treated with 1µg all-trans retinoic acid and reincubated. At stage 20 (day 3) and stage 24 (day 4), dorsal aortic flow velocities were measured with a 20-MHz pulsed Doppler velocity meter, in normal and retinoic-acid-treated embryos. Flow velocity waveforms were assessed both before and after the administration of epinephrine (5 or 10µg). Results: Epinephrine caused a significant increase (p < 0.05) in heart rate, peak and mean velocities, peak acceleration, peak and mean blood flows, stroke volume and dorsal aortic area of both stage 20 and stage 24 normal and retinoic-acid-treated chick embryos. However, before epinephrine administration, stage 24 retinoic-acidtreated embryos displayed a significantly lesser increase in all outcome variables with the exception of dorsal aortic area. This was even observed after epinephrine administration. The effect of retinoic acid on cardiac output could not be compensated by epinephrine application. Conclusion: Epinephrine affects hemodynamics in both normal embryos and retinoic-acid-treated embryos prior to sympathetic innervation. A significant difference in hemodynamics exists between stage 24 normal and retinoic- acid-treated embryos. The underlying mechanism for the observed hemodynamic changes will need to be investigated.
- Subjects
ADRENALINE; HEMODYNAMICS; BLOOD circulation; RETINOIDS; CHICKEN embryos
- Publication
Fetal Diagnosis & Therapy, 2004, Vol 19, Issue 5, p431
- ISSN
1015-3837
- Publication type
Article
- DOI
10.1159/000078996