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- Title
Kangaroo vs. Porcine Aortic Valves: Calcification Potential after Glutaraldehyde Fixation.
- Authors
Narine, K.; Chéry, Cyrille C.; Goetghebeur, Els; Forsyth, R.; Claeys, E.; Cornelissen, Maria; Moens, L.; van Nooten, G.
- Abstract
The aim of this study was to evaluate and compare the calcification potential of kangaroo and porcine aortic valves after glutaraldehyde fixation at both low (0.6%) and high (2.0%) concentrations of glutaraldehyde in the rat subcutaneous model. To our knowledge this is the first report comparing the time-related, progressive calcification of these two species in the rat subcutaneous model. Twenty-two Sprague-Dawley rats were each implanted with two aortic valve leaflets (porcine and kangaroo) after fixation in 0.6% glutaraldehyde and two aortic valve leaflets (porcine and kangaroo) after fixation in 2% glutaraldehyde respectively. Animals were sacrificed after 24 h and thereafter weekly for up to 10 weeks after implantation. Calcium content was determined using inductively coupled plasma-mass spectrometry and confirmed histologically. Mean calcium content per milligram of tissue (dry weight) treated with 0.6 and 2% glutaraldehyde was 116.2 and 110.4 μg/mg tissue for kangaroo and 95.0 and 106.8 μg/mg tissue for porcine valves. Calcium content increased significantly over time (8.8 μg/mg tissue per week) and was not significantly different between groups. Regression analysis of calcification over time showed no significant difference in calcification of valves treated with 0.6 or 2% glutaraldehyde within and between the two species. Using the subcutaneous model, we did not detect a difference in calcification potential between kangaroo and porcine aortic valves treated with either high or low concentrations of glutaraldehyde. Copyright © 2005 S. Karger AG, Basel
- Subjects
HEART valves; NUCLEAR spectroscopy; REGRESSION analysis; HEART; AORTIC valve; MITRAL valve
- Publication
European Surgical Research, 2005, Vol 37, Issue 3, p137
- ISSN
0014-312X
- Publication type
Article
- DOI
10.1159/000085960