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- Title
Exosomal miRNA profile as complementary tool in the diagnostic and prediction of treatment response in localized breast cancer under neoadjuvant chemotherapy.
- Authors
Rodríguez-Martínez, Alba; de Miguel-Pérez, Diego; Ortega, Francisco Gabriel; García-Puche, José Luis; Robles-Fernández, Inmaculada; Exposito, José; Martorell-Marugan, Jordi; Carmona-Sáez, Pedro; Garrido-Navas, María del Carmen; Rolfo, Christian; Ilyine, Hugh; Lorente, José Antonio; Legueren, Marta; Serrano, María José
- Abstract
<bold>Background: </bold>Breast cancer patients under neoadjuvant chemotherapy includes a heterogeneous group of patients who eventually develop distal disease, not detectable by current methods. We propose the use of exosomal miRNAs and circulating tumor cells as diagnostic and predictive biomarkers in these patients.<bold>Methods: </bold>Fifty-three breast cancer women initially diagnosed with localized breast cancer under neoadjuvant chemotherapy were prospectively enrolled in this study. However, six of them were later re-evaluated and diagnosed as metastatic breast cancer patients by PET-CT scan. Additionally, eight healthy donors were included. Circulating tumor cells and serum exosomal miRNAs were isolated from blood samples before and at the middle of neoadjuvant therapy and exosomal miRNA levels analyzed by qPCR.<bold>Results: </bold>Before neoadjuvant therapy, exosomal miRNA-21 and 105 expression levels were higher in metastatic versus non-metastatic patients and healthy donors. Likewise, higher levels of miRNA-222 were observed in basal-like (p = 0.037) and in luminal B versus luminal A (p = 0.0145) tumor subtypes. Exosomal miRNA-222 levels correlated with clinical and pathological variables such as progesterone receptor status (p = 0.017) and Ki67 (p = 0.05). During neoadjuvant treatment, exosomal miRNA-21 expression levels directly correlated with tumor size (p = 0.039) and inversely with Ki67 expression (p = 0.031). Finally, higher levels of exosomal miRNA-21, miRNA-222, and miRNA-155 were significantly associated with the presence of circulating tumor cells.<bold>Conclusion: </bold>Liquid biopsies based on exosomal miRNAs and circulating tumor cells can be a complementary clinical tool for improving breast cancer diagnosis and prognosis.
- Subjects
METASTATIC breast cancer; BREAST cancer; CANCER patients; BLOOD sampling; BREAST cancer prognosis; MICRORNA; TREATMENT effectiveness; CANCER cells; BREAST cancer diagnosis
- Publication
Breast Cancer Research, 2019, Vol 21, Issue 1, pN.PAG
- ISSN
1465-5411
- Publication type
journal article
- DOI
10.1186/s13058-019-1109-0