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- Title
The essential prerequisites for quantitative RT-PCR.
- Authors
Halford, William P.
- Abstract
Focuses on the issue of quantitative application of the reverse transcription-polymerase chain reaction (RT-PCR). Theoretical constraints which caused the questioning of the feasibility of RT-PCR; Provisions of competitive PCR for investigators; Limitations of competitive RT-PCR; Bases of the assertion that competitive DNAs are essential to quantitative PCR.
- Subjects
REVERSE transcriptase; POLYMERASE chain reaction; DNA
- Publication
Nature Biotechnology, 1999, Vol 17, Issue 9, p835
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/12783