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- Title
Antibody Dependent Cellular Cytotoxicity and Mitogen Responsiveness of Human Peripheral Blood Lymphocytes Differing in Avidity for Sheep Erythrocytes.
- Authors
Jónsdóttir, I.; Dillner-Centerlind, M.-L.; Perlmann, H.; Perlmann, P.
- Abstract
To characterize the effector cells (K-cells) in antibody-dependent cellular cytotoxicity (ADCC) against a nucleated target cell (El 4), human peripheral blood lymphocytes (PBL) were fraction- ated by rosette sedimentation into subpopulations differing in avidity for sheep erythrocytes (E), The fractions obtained were assayed for surface markers, and as a functional T cell marker, for responsiveness to the mitogen leucoagglutinin (La). By depleting PBL of E-receptor-bearing cells (Et+), approximately half of the cytotoxic potential was found in the Et-depleted friction. While the Et+ fraction had low activity on t per cell basis, it nevertheless contained a significant proportion of the original cytotoxicity. By sequential E-rosetting, fractions binding E with high avidity (Et+), low avidity (EL+) or not at nil (E-) were obtained. Both E-binding fractions consisted primarily of T-cells, as judged from their surface marker profiles. Both fractions responded well to La, but with different dote optima. The E- friction contained primarily B- and null ceils and did not respond to La. Significant K-cell activity was found in nil these frictions. The results show that a significant friction of the K-cells have receptors for E and these can he of either high or low avidity. Since both EH+ and EL+ fractions respond well to La and contain K-cells, a T-cell origin of the hatter is suggested. However, whether or not cyto- toxicity and La-responsiveness are functions of the same cells remains to be established.
- Subjects
ANTIBODY-dependent cell cytotoxicity; MITOGENS; T cells; LYMPHOCYTES; KILLER cells; CELLULAR immunity
- Publication
Scandinavian Journal of Immunology, 1979, Vol 10, Issue 6, p525
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1979.tb01386.x