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- Title
Serum Factors from Patients with Systemic Lupus Erythematosus Enhancing Superoxide Generation by Normal Neutrophils.
- Authors
Shingu, Masao; Oribe, Motohiro; Todoroki, Takashi; Tatsukawa, Keizi; Tomo-Oka, Kazuhisa; Yasuda, Masayuki; Nobunaga, Masashi
- Abstract
It has been suggested that human neutrophils exposed to preformed immune complexes or activated complement fragments generate O2- anions in extracellular medium. In vivo studies have revealed that oxygen intermediates produced by immune complex-activated neutrophils play an important role in subsequent tissue damage. Since it is difficult to obtain direct evidence that O2- is released into plasma in patients with systemic lupus erythematosus (SLE), we studied the capacities of their sera to stimulate O2- release by human neutrophils in vitro. Sera from patients with SLE significantly enhanced O2- generation by neutrophils compared to normal sera. The enhancing activity of serum in the induction of increased O2- generation correlated positively with the presence of serum immune complexes and negatively with serum complement levels. The enhancing factors were analyzed by serum fractionation on Sephadex G-200 gel filtration, and were concluded to be immune complexes of intermediate size containing an activated complement fragment.
- Subjects
LUPUS erythematosus; NEUTROPHILS; PHAGOCYTES; ANIONS; IMMUNOGLOBULINS; SKIN diseases
- Publication
Journal of Investigative Dermatology, 1983, Vol 81, Issue 3, p212
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12517989