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- Title
Early apoptosis in CD34<sup>+</sup> cells as a potential heterogeneity in quality of cryopreserved umbilical cord blood.
- Authors
Shim, Jae-Seung; Cho, Bin; Kim, Myungshin; Park, Gyeong-Sin; Shin, Jong-Chul; Hwang, Hong-Kyu; Kim, Tae-Gyu; Oh, Il-Hoan
- Abstract
The increased use of umbilical cord blood (UCB) raises issues regarding the quality of cryopreserved UCB. This study investigated whether early apoptosis of CD34+ cells is part of the functional heterogeneity of cryopreserved UCB. Annexin V binding of CD34+PI(−) cells showed wide variations in both fresh and cryopreserved UCBs, with greater variation among units frozen for >5 years. Xenotransplantation of sorted cells into non-obese diabetic severe combined immunodeficient mice demonstrated that the Annexin V assay identified most repopulating activities in UCB units. Thus, early apoptosis of CD34+ cells could influence the outcome of transplantation using cryopreserved UCB.
- Subjects
HEMATOPOIETIC stem cells; APOPTOSIS; CORD blood; HETEROGENEITY; BLOOD diseases
- Publication
British Journal of Haematology, 2006, Vol 135, Issue 2, p210
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1111/j.1365-2141.2006.06270.x