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- Title
Differential expression profile of membrane proteins in Aplysia pleural-pedal ganglia under the stress of methyl parathion.
- Authors
Chen, Ying-Ying; Huang, Lin; Zhang, Yong; Ke, Cai-Huan; Huang, He-Qing
- Abstract
This study was aimed to analyze the alteration of membrane protein profiles in Aplysia juliana Quoy & Gaimard ( A. juliana) pleural-pedal ganglia under MP exposure. Both the results of GC-MS analysis and the activity assay of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT) reveal that MP toxicological effects on Aplysia left and right pleural-pedal ganglia are different under 7 and 14 days of exposure. Therefore, Aplysia were subjected for exposure at two concentrations (1 and 2 mg/l) of MP for 7 and 14 days for membrane proteomic study. As a result, 19 and 14 protein spots were differentially expressed in A. juliana left pleural-pedal ganglia under 7 and 14 days treatment, and 20 and 14 protein spots found with differential expressions in their right ganglia under the same treatment, respectively. Several proteins with expression variations were detected from both the left and right pleural-pedal ganglia; however, most proteins have distinctive expressions, indicating different mechanisms might be involved in initiating MP toxicology in left and right ganglia. Among the total differential protein spots obtained, 29 proteins were classed as membrane proteins. These proteins are mainly involved in the metabolism process, cell redox homeostasis, signal transduction, immunology, intracellular transport and catalysis, indicating MP toxicity in mollusks seems to be complex and diverse. Some differentially expressed proteins were further confirmed by Western blotting and quantitative real-time PCR. These results might provide renovated insights to reveal the mechanism of MP-induced neurotoxicity, and the novel candidate biomarkers might have potential application for environmental evaluation of MP pollution level.
- Subjects
MEMBRANE proteins; APLYSIA; METHYL parathion; GANGLIA; SUPEROXIDE dismutase; ACETYLCHOLINESTERASE
- Publication
Environmental Science & Pollution Research, 2014, Vol 21, Issue 5, p3371
- ISSN
0944-1344
- Publication type
Article
- DOI
10.1007/s11356-013-2210-z