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- Title
DEVELOPMENT OF A MODEL SYSTEM TO COMPARE CELL SEPARATION METHODS FOR THE ISOLATION OF FETAL CELLS FROM MATERNAL BLOOD.
- Authors
BIANCHI, DIANA W.; KLINGER, KATHERINE W.; VADNAIS, THERESA J.; DEMARIA, MARY ANN; SHUBER, ANTHONY P.; SKOLETSKY, JOEL; MIDURA, PAT; DIRISO, MATTHEW; PELLETIER, CHRISTINE; GENOVA, MICHELLE; ERIKSON, MARLENA S.; WILLIAMS, JOHN M.
- Abstract
Three major methods have been described for the isolation of fetal cells from maternal blood: fluorescence-activated cell sorting (FACS), immunomagnetic beads, and magnetic-activated cell sorting (MACS). To date, no study has directly compared fetal cell recovery using each of these methods. Here we describe our system using a 'model' male fetal cell mixed into female peripheral blood mononuclear cells. Fetal cell yields and purities were assayed by a quantitative polymerase chain reaction (qPCR) using chromosomes Y- and 7-specific sequences. Fetal cell recovery was investigated by selection of CD71+ cells or depletion of CD45+ cells. Our data demonstrated variation in fetal cell recovery for all methods tested, although CD71+ selection by FACS gave the best and most consistent results.
- Publication
Prenatal Diagnosis, 1996, Vol 16, Issue 4, p289
- ISSN
0197-3851
- Publication type
Article
- DOI
10.1002/(SICI)1097-0223(199604)16:4<289::AID-PD843>3.0.CO;2-T