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- Title
Hsa_circ_0079598 acts as a potential diagnostic and prognostic biomarker for gastric cancer.
- Authors
Xu, Huiting; Cao, Xiaoli; Zhang, Xiaoxia; Xue, Yajing; Chen, Bingyan; Wang, Feng; Sun, Yajun
- Abstract
The work discussed the expression level circular RNA (circRNA) hsa_circ_0079598 for gastric cancer (GC) patients as well as clinicopathological significance. qRT-PCR was utilized for determining the expression level of hsa_circ_0079598 in GC cell lines, 40 GC tissue pairs and their paracancerous tissue specimens, 60 pairs of preoperative and postoperative plasma specimens of GC patients, 20 plasma specimens of patients with gastric polyps, and 60 plasma specimens of the healthy control group. Besides, the correlation with clinicopathological parameters was analyzed. Tumor indices glycan antigen 199 (CA199), glycan antigen 724 (CA724), and pepsinogen I (PGI) were combined to evaluate their auxiliary diagnostic value. The values of hsa_circ_0079598 in the prognosis of GC patients were analyzed using survival curves. The relative expression of hsa_circ_0079598 in the cancerous tissues of GC patients was lower than that of their paracancerous tissues (p=0.004). The relative expression of plasma hsa_circ_0079598 in GC patients was lower than that of the healthy control group (p<0.001) and patients with gastric polyps. Moreover, the relative expression of human gastric epithelial cell lines (GES-1) (p<0.05) was higher than that of hsa_circ_0079598 of HGC-27, AGS, and MGC-803 cancer cell lines in GC cell lines. The total survival time of the high-expression patient group (p=0.040) was higher than that of hsa_circ_0079598 in the low-expression patient group. The expression of hsa_circ_0079598 in patients with the tissues and plasma of GC was related to the differentiated degree of tumors and TNM staging (p<0.05). The auxiliary diagnostic value of hsa_circ_0079598 for GC was analyzed using ROC curves. When GC was distinguished from gastric polyps and the healthy control group, the areas under the ROC curve (AUCs) were 0.856 and 0.930, respectively. Hsa_circ_0079598 combined with tumor indices CA199, CA724, and PGI detection had higher specificity and accuracy than those of a single index. Hsa_circ_0079598 was low-expression in cell lines, plasma, and GC tissues, which was related to the progress and prognosis of GC. Hsa_circ_0079598 combined with CA199, CA724, and PGI detection can improve the diagnosis of GC.
- Subjects
STOMACH cancer; GENE expression; CIRCULAR RNA; BIOMARKERS; RECEIVER operating characteristic curves
- Publication
Oncologie (De Gruyter), 2023, Vol 25, Issue 2, p179
- ISSN
1765-2839
- Publication type
Article
- DOI
10.1515/oncologie-2023-0047