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- Title
Effects of HMGA2 si RNA and doxorubicin dual delivery by chitosan nanoparticles on cytotoxicity and gene expression of HT-29 colorectal cancer cell line.
- Authors
Siahmansouri, Homayoon; Somi, Mohammad Hossein; Babaloo, Zohreh; Baradaran, Behzad; Jadidi‐Niaragh, Farhad; Atyabi, Fatemeh; Mohammadi, Hamed; Ahmadi, Majid; Yousefi, Mehdi
- Abstract
Objective Over-expressions of HMGA2, vimentin and MMP-9 and downregulation of E-cadherin occur on colorectal cancer cells followed by a reduction in let-7 as a regulatory factor. In this study, we first used carboxymethyl dextran ( CMD)-chitosan nanoparticles (Ch NPs) platform to encapsulate HMGA2 si RNA and doxorubicin ( DOX), and then, we evaluated the efficacy of the simultaneous delivery of si RNA/drug on viability and gene expression of HT-29 cell lines. Methods Ch NPs characteristics were determined by a dynamic light scattering and zeta sizer. Morphology of loaded Ch NPs was assessed by scanning electron microscopy, and Fourier transform infrared spectroscopy was used to confirm the conjugation of Ch NP/si RNA/ DOX/ CMD. Cell viability and relative mRNA expression were evaluated by MTT assay and real-time PCR, respectively. Key finding The prepared Ch NPs had high efficiency for si RNA and drug encapsulation (78% and 75%) and were stable against serum and heparin. Ch NP/si RNA/ DOX/ CMD was more effective to induce tumour cell death and also could significantly reduce the expressions of HMGA2, vimentin as well as MMP-9 and increase E-cadherin expression. Conclusion In conclusion, our results revealed that dual delivery of a key gene si RNA and appropriate anticancer drug have great impact on the treatment of colorectal cancer.
- Subjects
CHITOSAN; COLON cancer treatment; NANOPARTICLES; CELL-mediated cytotoxicity; GENE expression; DOXORUBICIN; VIMENTIN; ANTINEOPLASTIC agents
- Publication
Journal of Pharmacy & Pharmacology, 2016, Vol 68, Issue 9, p1119
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/jphp.12593