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- Title
Enzyme immunoassay of usnic acid in lichens.
- Authors
Burkin, A.; Kononenko, G.; Tolpysheva, T.
- Abstract
An enzyme immunoassay for usnic acid in lichens was developed, the sensitivity of which was 0.1 μg/g of air-dried material (0.00001%). Polyclonal rabbit antibodies against bovine serum albumin conjugated to (+)-usnic acid under the conditions of formaldehyde condensation made it possible to determine the analyzed substance in solutions at concentrations from 1 ng/mL when it interacts with immobilized gelatin conjugate homologous in the binding mode. Usnic acid in 2-26600 μg/g (0.0002-2.6%) amounts was found in all 236 studied samples of lichens belonging to 53 species and 8 families.
- Subjects
ENZYME-linked immunosorbent assay; LICHENS; SERUM albumin; IMMOBILIZED proteins; GELATIN; FORMALDEHYDE; IMMUNOGLOBULINS
- Publication
Applied Biochemistry & Microbiology, 2013, Vol 49, Issue 3, p315
- ISSN
0003-6838
- Publication type
Article
- DOI
10.1134/S000368381303006X