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- Title
Anther culture of japonica rice Kenyu 28×Nanjing 44 hybrid F<sub>1</sub> and its regenerated plantlets identification.
- Authors
HU Ting-ting; WANG Jian-kang; DING Cheng-wei; GUO Rong-liang; WU Yu-ling; XU Jia-an; WANG You-shuang; ZHAO Yi-peng
- Abstract
[Objective]The present study was conducted to obtain new stable intervarietal hybrid materials of japonica rice varieties quickly in order to take full advantage of intervarietal heterosis of japonica rice varieties. [Method]The anthers of Kenyu 28×Nanjing 44 hybrid F1 plants were cultured to get DH plantlets, and their main agronomic traits , ploidy and resistances to rice blast disease were identified and evaluated. [Result] The cultured an - thers began to dehisce after inoculating into callus induction medium for 5 days , and the yellow -green callus was found to be 2 - 3 mm in length after induction of 10-15 days. By transfering into differentiation medium, the callus could grow up plantlets after induction of 20 days in MS medium supplemented with 6 - BA 1.0 mg/L, NAA 0.2 mg/L, MET 2.0 mg/L, sugar 30.0 g/L, LH 0.5 g/L and agar powder 8.0 g/L. The 1696 anthers could induce callus, and the induction rate was 66.5%. Out of 382 anther culture regenerated plantlets, the haploid, diploid, tetraploid plantlets accounted for 27.5%, 70.2% and 2.3% , respectively. Different ploidy plantlets were found to present trait variations viz. , plant dwarf, barrenness, significant decrease of tiller and chloroplast number in guard cell. Each three diploid materials with good comprehensive characters were found to be highly resistant or moderately resistant to rice blast disease through identifying in the field. Out of them, 2 diploid materials presented good comprehensive characters, high seed setting rate (89.6% - 90.1%) , significant decrease of plant height and resistance to rice blast disease. [Conclusion] Stable intermediate materials can be obtained quickly through anther culture of japonica rice intervarietal hybrid F1, which is feasible method for creating and breeding new materials with resistance to disease and so on.
- Publication
Journal of Southern Agriculture, 2014, Vol 45, Issue 11, p1910
- ISSN
2095-1191
- Publication type
Article
- DOI
10.3969/j:issn.2095-1191.2014.11.1910